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Karlsruhe Institute of Technology

Young Investigator Network
YIN-Office

Engler-Bunte-Ring 21
76131 Karlsruhe

 

Tel. +49 721 608-46184

E-Mail: infoZnu6∂yin kit edu

 

Upcoming Events
Tuesday, 18 Jun 2019
YIN Lunch Meeting at CN
Thursday, 27 Jun 2019
Writing successful ERC starting grant applications
Wednesday, 10 Jul 2019
NEULAND - Der Innovationstag am KIT
Tuesday, 24 Sep 2019
2-Day Workshop "The Art of Presenting Oneself"
Friday, 11 Oct 2019
YIN Day 2019

Weitere Veranstaltungen...
Das KIT ist seit 2010 als familiengerechte Hochschule zertifiziert.
Dr. Lars Wegner

Dr. Lars Wegner

Business Consultant
Biotechnologie, Elektrophysiologie, Fluoreszierende Mikroskopie
Room: Deutschland, Karlsruhe
lars wegnerVek1∂gmx net

 



Physiological effects of pulsed electromagnetic fields on plant cells

Junior Research Group of Dr. Lars H. Wegner

 

The 'Shared Research Group' (SRG) 60-1

This shared research group emanates from a co-operation of the Botanical Institute I (Karlsruhe University) and of the Department of Pulsed Power and Microwave Technology (IHM; Karlsruhe Research Center); it deals with the effects of pulsed electric fields on plants and plant cells. Cells are enclosed by a lipid membrane that serves as a diffusion barrier, separating the cell interior from its environment. By exposing cells to high-energy electric field pulses this membrane can be made transiently permeable to introduce macromolecules like DNA into these cells (so-called “electroporation”). Basic research on plant cells is performed in order to make this process more efficient.

 

The techniques we use:

  • Plant cell culture

  • Pulsed power equipment, “electroporator”

  • Patch clamp technique

  • Voltage-sensitive dyes to measure plant

  • Membrane potentials

  • Confocal fluorescent microscopy

 

Our current projects:

  • Calibration of voltage-sensitive dyes by combining fluorescent microscopy and patch clamp; test for adverse side effects of the dye on cell physiology (e.g. on ion channel activity).

  • Macromolecules labelled with a fluorescent dye are used as a tool to study electroporation.

  • Application of the patch clamp technique to plant (cell) physiology (interaction with pathogens, salt tolerance).

  • Design of a new, gentle technology to measure current-voltage relations of plant cells and yeast by combining optical and electrical methods.