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Karlsruhe Institute of Technology

Young Investigator Network
YIN-Office

Engler-Bunte-Ring 21
76131 Karlsruhe

 

Tel. +49 721 608-46184

E-Mail: infoIqt2∂yin kit edu

 

Das KIT ist seit 2010 als familiengerechte Hochschule zertifiziert.
Dr. Lars Wegner

Dr. Lars Wegner

Business Consultant
Biotechnologie, Elektrophysiologie, Fluoreszierende Mikroskopie
Room: Deutschland, Karlsruhe
lars wegnerTsi1∂gmx net

 



Physiological effects of pulsed electromagnetic fields on plant cells

Junior Research Group of Dr. Lars H. Wegner

 

The 'Shared Research Group' (SRG) 60-1

This shared research group emanates from a co-operation of the Botanical Institute I (Karlsruhe University) and of the Department of Pulsed Power and Microwave Technology (IHM; Karlsruhe Research Center); it deals with the effects of pulsed electric fields on plants and plant cells. Cells are enclosed by a lipid membrane that serves as a diffusion barrier, separating the cell interior from its environment. By exposing cells to high-energy electric field pulses this membrane can be made transiently permeable to introduce macromolecules like DNA into these cells (so-called “electroporation”). Basic research on plant cells is performed in order to make this process more efficient.

 

The techniques we use:

  • Plant cell culture

  • Pulsed power equipment, “electroporator”

  • Patch clamp technique

  • Voltage-sensitive dyes to measure plant

  • Membrane potentials

  • Confocal fluorescent microscopy

 

Our current projects:

  • Calibration of voltage-sensitive dyes by combining fluorescent microscopy and patch clamp; test for adverse side effects of the dye on cell physiology (e.g. on ion channel activity).

  • Macromolecules labelled with a fluorescent dye are used as a tool to study electroporation.

  • Application of the patch clamp technique to plant (cell) physiology (interaction with pathogens, salt tolerance).

  • Design of a new, gentle technology to measure current-voltage relations of plant cells and yeast by combining optical and electrical methods.